Characteristics of scoria adsorption of Escherichia coli phage in groundwater

doi:10.13745/j.esf.sf.2021.2.13

Abstract

Abstract:

To understand the characteristics of scoria adsorption removal of Escherichia coli phage from groundwater, we investigated the effect of the Escherichia phage vB_EcoM-ep3 adsorption on scoria in groundwater through laboratory experiments. Using the same amount of zeolite and quartz sand as a contrast, we further analyzed the influence of hydrochemical factors in groundwater (Fe ²⁺, Fe³⁺, Mn²⁺, $SO 4 2 -$ , $NH 4 +$ , $NO 3 -$ , $NO 2 -$ , Ca²⁺, Mg²⁺, $CO 3 2 -$ , $HCO 3 -$ , pH) on the adsorption process. The biological characteristics of Escherichia coli phage and its microstructural change before and after scoria adsorption were analyzed by means of microscopic detection technology. The results showed that the scoria adsorption efficiency was higher than zeolite and quartz sand, with the maximum adsorbance reaching 2.245×10 ⁵ pfu/g. The influence of each hydrochemical factors on scoria adsorption varied. Fe²⁺ and Fe³⁺ promoted scoria adsorption, while $SO 4 2 -$ , nitrogen species and high alkalinity inhibited the adsorption process. The low Mn²⁺ ( $C M n 2 +$ , 10 mg/L) and Ca²⁺( $C C a 2 +$ , 100 mg/L) concentrations slightly promoted adsorption; beyond this concentration, the inhibitory effect was shown. The pH value had a great influence on scoria adsorption characteristics. Higher pH corresponded to weaker adsorption effect. The vB_EcoM-ep3 phage has an equilateral hexagonal head with a body diameter of about (53±2) nm, and a tail made of stretchable tail fiber with a length of about (107±3) nm. Upon adsorption the immobilized phage occupied most of the surface pores in the scoria honeycomb structure.

Key words: scoria, groundwater, Escherichia coli phage, adsorption, characterization

CLC Number:

X523

ZHANG Yuling, YIN Siqi, SI Chaoqun, WANG Xi, CHU Wenlei. Characteristics of scoria adsorption of Escherichia coli phage in groundwater[J]. Earth Science Frontiers, 2021, 28(5): 167-174.

Figures/Tables 13

Fig.1 Adsorption materials

Fig.2 Adsorption efficiency of three kinds of materials

Fig.3 Scoria adsorption dynamics

Fig.4 Kinetics model fitting for scoria adsorption

Fig.5 Influence of Fe2+ and Fe3+ on scoria adsorption efficiency

Fig.6 Influence of Mn2+ on scoria adsorption efficiency

Fig.7 Influence of S O 4 2 - on scoria adsorption efficiency

Fig.8 Influence of nitrogen species on scoria adsorption efficiency

Fig.9 Influence of Ca2+ and Mg2+ on scoria adsorption efficiency

Fig.10 Influence of CO 3 2 - and HCO 3 - on scoria adsorption efficiency

Fig.11 Influence of pH value on scoria adsorption efficiency

Fig.12 Structure of Escherichia phage vB_EcoM-ep3

Fig.13 Change of scoria microstructure before (a) and after (b) vB_EcoM-ep3 adsorption

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