Degradation of 1,2,4-trichlorobenzene by an anaerobic enrichment culture mediated by Dehalobacter species

doi:10.13745/j.esf.sf.2022.10.41

Abstract

Abstract:

1,2,4-Trichlorobenzene (TCB) is a typical organic contaminant in soil and groundwater at industrial plant in China. 1,2,4-TCB is recalcitrant, bio-accumulative and highly toxic, posing serious threats to the environment and human health. 1,2,4-TCB is denser than water and easily penetrates into an anaerobic environment. Therefore, understanding the anaerobic biotransformation of 1,2,4-TCB is of great practical significance to on-site remediation. In this study, an anaerobic enrichment culture, which can sustainably dechlorinate 1,2,4-TCB to MCB via 1,4-DCB, is obtained. 16S rRNA gene amplicon sequencing and qPCR demonstrated Dehalobacter is responsible for the observed reductive dechlorination with a growth yield of (1.68±0.8)×10⁶ copies per μmol released Cl^-. A complete 16S rRNA gene sequence was obtained through PCR and a corresponding phylogenetic tree was constructed. This study provides a reference for the enrichment culture technique as well as theoretical guidance for the in-situ anaerobic bioremediation of contaminated sites.

Key words: 1,2,4-trichlorobenzene, groundwater contamination, bioremediation, Dehalobacter, reductive dechlorination

CLC Number:

X523
X172

LÜ Lianghua, QIAO Wenjing, ZHANG Han, YE Shujun, WU Jichun, WANG Shui, JIANG Jiandong. Degradation of 1,2,4-trichlorobenzene by an anaerobic enrichment culture mediated by Dehalobacter species[J]. Earth Science Frontiers, 2024, 31(2): 472-480.

Figures/Tables 6

Fig.1 The dechlorination of 1,2,4-trichlorobenzene in three active bottles

Fig.2 The microbial community composition of the 1,2,4-trichlorobenzene-dehalogeanting enrichment culture

Fig.3 The qPCR results of the total bacteria and Dehalobacter

Table 1 Growth yield of obligate organohalide-respiring bacterium Dehalobacter

培养周期	平行样	释放的氯离子量/ μmol	Dehalobacter细胞数量/ (copies·bottle^-1)	Dehalobacter生长率(释放的氯离子)/ (copies·μmol^-1)
第0~30天	#1	32.11	5.01×10⁶	1.58×10⁵
	#2	31.73	8.78×10⁶	2.78×10⁵
	#3	24.97	1.47×10⁶	6.23×10⁴
第31~90天	#1	147.95	3.34×10⁸	2.18×10⁶
	#2	110.69	2.40×10⁸	2.09×10⁶
	#3	103.69	8.19×10⁷	7.61×10⁵

Fig.4 Electrophoretic diagram of the PCR products

Fig.5 The phylogenetic tree of Dehalobacter spp.

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