Effect of humic acid on the phase distribution of sulfadiazine in shallow groundwater

doi:10.13745/j.esf.sf.2021.2.20

Abstract

Abstract:

It is important for exploring the behavioral characteristics of antibiotics and assessing their hazards by studying the distribution characteristics of antibiotics in shallow groundwater. In this paper, the interaction of sulfadiazine (SD) with humic acid (HA) and the effect of HA on the phase distribution of SD in shallow groundwater were studied by three-dimensional fluorescence quenching method. The effects of HA concentration, pH and temperature on SD in different phases were discussed, and the distribution coefficient as well as quenching and thermodynamic parameters for the quenching process were calculated. The results showed that both low SD (0.5 mg/L) and elevated HA (0-20 mg/L) can significantly increase SD partition in the suspension phase. The mechanism of HA fluorescence quenching by SD was to form HA·SD complex via a single static process. The number of binding site was 1; the quenching constant K_sv was 5.271×10³ L/mol; and the quenching rate constant K_q was 5.271×10 ¹¹ L/(mol·s). It showed the HA-SD interaction was a spontaneous exothermic process. In a low-temperature (15 ℃), neutral or weakly alkaline (pH 8-10) environment, antibiotics had the strongest quenching effect on HA, and, accordingly, HA had the most obvious promoting effect on SD entering the suspension phase.

Key words: three-dimensional fluorescence method, humic acid, sulfadiazine, interaction, distribution between phases

CLC Number:

X523

DUAN Lei, YANG Shengke, WANG Wenke. Effect of humic acid on the phase distribution of sulfadiazine in shallow groundwater[J]. Earth Science Frontiers, 2021, 28(5): 104-113.

Figures/Tables 12

Fig.1 Particle size distribution in suspended matter

Fig.2 Three-dimensional fluorescence image of HA

Fig.3 Sulfadiazine partition between phases at equilibrium (left) and SD phase distribution coefficient Kd(right) as a function of SD initial concentration

Fig.4 Effect of HA on SD phase distribution

Fig.5 Humic acid and SD distributions in the suspension phase

Fig.6 Humic acid fluorescence quenching curves for varying SD concentrations at 25 ℃ and pH 7

Fig.7 Stern-Volmer curve of HA fluorescence quenching by SD at 25 ℃

Fig.8 Stern-Volmer curve of HA fluorescence quenching by SD for different temperatures

Fig.9 Humic acid fluorescence quenching by SD at varying pH

Fig.10 Stern-Volmer curve of HA fluorescence quenching by SD for varying pH

Table 1 Thermodynamic parameters for SD-HA interaction at different temperatures

抗生素	pH 值	K_sv/ (10³L·mol^-1)	K_q/ (10¹¹L·mol^-1·s^-1)	K_b/ (10³L·mol^-1)	n
SD	4	3.894	3.894	3.724	1.34
	8	4.043	4.043	4.015	0.69
	10	3.946	3.946	3.824	0.81

Fig.11 Fourier infrared spectrum of SD, HA or HA-SD

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