Effects of o-nitro-p-methylphenol and o-amino-p-methylphenol on the anaerobic biodegradation of 1,2,4-TCB

doi:10.13745/j.esf.sf.2021.2.16

Abstract

Abstract:

In a Nanjing chemical plant manufacturing o-nitro-p-methylphenol and o-amino-p-methylphenol, field investigation and microcosm study found contaminated groundwater where the contaminant, 1,2,4-trichlorobenzene (1,2,4-TCB), was biodegraded by indigenous anaerobic microbes. In this study, we conducted three groups of microcosm experiments to explore the effects of o-nitro-p-methylphenol and o-amino-p-methylphenol on the 1,2,4-TCB dechlorination process, using (1) 1,2,4-TCB alone, (2) 1,2,4-TCB and o-amino-p-methylphenol, or (3) 1,2,4-TCB and o-nitro-p-methylphenol as the substrate(s), and compared the degradation processes, degradation rates, and microbial communities for the three groups of experiments. The results show: (1) O-nitro-p-methylphenol was reduced to o-amino-p-methylphenol while 1,2,4-TCB dechlorination was inhibited, with 1,4-DCB as the only dechlorination product. (2) O-amino-p-methylphenol did not prohibit 1,2,4-TCB degradation but decreased degradation rate. (3) Azospira and Pseudomonas were involved in o-nitro-p-methylphenol reduction, especially Pseudomonas was probably the functional microorganism under the anaerobic condition at the site.

Key words: o-nitro-p-methylphenol, o-amino-p-methylphenol, 1,2,4-TCB, anaerobic biodegradation, microbial community

CLC Number:

LIU Zhitong, ZHOU Ni, QIAO Wenjing, YE Shujun. Effects of o-nitro-p-methylphenol and o-amino-p-methylphenol on the anaerobic biodegradation of 1,2,4-TCB[J]. Earth Science Frontiers, 2021, 28(5): 159-166.

Figures/Tables 10

References 21

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化学名称	分子式	化学结构式	分子量	外观	化学性质
邻氨基对甲基苯酚	C₇H₉NO		123.15	白色结晶粉末,遇空气易氧化变色	有刺激性。易溶于氯仿、乙醚、乙醇等有机溶剂。最大溶解度9 983 mg·L^-1 (25 ℃)
邻硝基对甲基苯酚	C₇H₆NO₃		152.13	黄色结晶块状或浅棕色油状液体	有强烈的刺激性气味。易溶于乙醇、乙醚、氯仿等有机溶剂。微溶于水,最大溶解度695 mg·L^-1(25 ℃)

化学名称	分子式	化学结构式	分子量	外观	化学性质
邻氨基对甲基苯酚	C₇H₉NO		123.15	白色结晶粉末,遇空气易氧化变色	有刺激性。易溶于氯仿、乙醚、乙醇等有机溶剂。最大溶解度9 983 mg·L^-1 (25 ℃)
邻硝基对甲基苯酚	C₇H₆NO₃		152.13	黄色结晶块状或浅棕色油状液体	有强烈的刺激性气味。易溶于乙醇、乙醚、氯仿等有机溶剂。微溶于水,最大溶解度695 mg·L^-1(25 ℃)

实验组	实验瓶编号	水土混合液用量/mL	地下水用量/mL	电子供体	电子受体
对照组	TCB1 TCB2 TCB3	10	150	无	1,2,4-TCB
TCB+ 邻氨基对甲基苯酚组	A1 A2 A3	10	150	无	1,2,4-TCB+ 邻氨基对甲基苯酚
TCB+ 邻硝基对甲基苯酚组	N1 N2 N3	10	150	无	1,2,4-TCB+ 邻硝基对甲基苯酚

实验组	实验瓶编号	水土混合液用量/mL	地下水用量/mL	电子供体	电子受体
对照组	TCB1 TCB2 TCB3	10	150	无	1,2,4-TCB
TCB+ 邻氨基对甲基苯酚组	A1 A2 A3	10	150	无	1,2,4-TCB+ 邻氨基对甲基苯酚
TCB+ 邻硝基对甲基苯酚组	N1 N2 N3	10	150	无	1,2,4-TCB+ 邻硝基对甲基苯酚

实验组	实验瓶编号	产物	44 d后TCB去除率/%	76 d后TCB去除率/%
TCB	TCB2	1,2-DCB; 1,3-DCB; 1,4-DCB; MCB	93.93	96.53
TCB	TCB3	1,2-DCB; 1,3-DCB; 1,4-DCB; MCB	94.26	97.51
TCB+ 邻硝基对甲基苯酚	N1	1,4-DCB; MCB	0	93.81
	N2	1,4-DCB; MCB	0	96.44
	N3	1,4-DCB; MCB	0	97.20
TCB+ 邻氨基对甲基苯酚	A2	1,4-DCB; MCB	93.38	96.24
TCB+ 邻氨基对甲基苯酚	A3	1,4-DCB; MCB	95.63	97.45