地学前缘 ›› 2015, Vol. 22 ›› Issue (1): 328-334.DOI: 10.13745/j.esf.2015.01.028

• 非主题来稿选登 • 上一篇    下一篇

Desulfovibrio sp.厌氧代谢淮南煤中>C12有机组分的实验研究

葛晓光，程健明，杨柳，叶永康，陈陆望   

  1. 1. 合肥工业大学 资源与环境工程学院, 安徽 合肥 230009
    2. 合肥工业大学 生物与食品工程学院, 安徽 合肥 230009
  • 收稿日期:2014-03-20 修回日期:2014-06-18 出版日期:2015-01-15 发布日期:2015-01-15
  • 作者简介:葛晓光(1956—)，男，博士，教授，主要从事地下水科学与工程研究。E-mail:xgge@hfut.edu.cn
  • 基金资助:

    国家自然科学基金项目(41172216，41372244)

 An experimental study of Desulfovibrio sp. anaerobic degradation of >C12 organic compounds of Huainan coal

  • Received:2014-03-20 Revised:2014-06-18 Online:2015-01-15 Published:2015-01-15

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摘要：

从淮南潘北煤矿采取530 m深石炭系太原组石灰岩钻孔出水水样和二叠系山西组煤样，开展煤的有机成分与煤系地层地下水的SRB代谢关系实验。从水样中分离纯化出一菌株S890，经基因测序鉴定为脱硫弧菌Desulfovibrio sp.。煤样的甲醇萃取物经气质联用GC-MS仪检测，匹配出21种主要有机化合物，均为C11以上的高级链烃、环烃、芳香烃及其衍生物。选择其中3种有机化合物2，6-Di-tert-butyl-4-methylphenol、2-hexyl-1-Decanol和Naphthalene，2，6-dimethyl做27-d的SRB细菌厌氧培养与底物SO2-4含量与H2S、FeS产生量对比观测试验，发现SRB细菌可有效利用前2种碳源，而Naphthalene，2，6-dimethyl未被利用。实验结果证实煤系地下水中的SRB生长可以有效地厌氧降解煤中一些较大分子有机化合物。

关键词: SRB, 煤系, 地下水, 有机物, 厌氧代谢

Abstract:

An experiment was made to study the anaerobic metabolism of organic components in coal by the SRBs (sulfate-reducing bacteria) using the groundwater samples of the Carboniferous Taiyuan Formation limestone aquifer containing bacteria and the coal samples of Shanxi Formation from mine-pit of 530 m depth at Panbei coal-mine in Huainan coal-field. A SRB strain S890 was cultured and isolated from the groundwater samples and was identified as Desulfovibrio sp. by S16 rRNA gene sequencing. The methanol extracts of the coal samples were analyzed with GC-MS, and 21 kinds of chief organic compounds were identified known as senior chain, cyclic and aromatic hydrocarbons as well as hydrocarbon derivatives. Three >C12 compounds (2,6-Di-tert-butyl4-methylphenon, 2-hexyl1-Decanol and Naphthalene,2,6-dimethyl) selected from the 21 compounds were measured as carbon sources in a 27-day SRB anaerobic cultivating experiment and concentration variation of SO2-4, H2S and FeS during the cultivating procedure in the controlled experiment, and the results reflected that 2,6-Di-tert-butyl4-methylphenon and 2-hexyl1-Decanol were evidently anaerobically degraded by Desulfovibrio sp., but Naphthalene,2,6-dimethyl was not. It was concluded from the experiment that the SRBs in groundwater in coal seams utilized some macromolecular organic compounds in the coals as carbon sources for their anaerobic growth.

Key words: SRB(sulfate reducing bacteria), coal seam, groundwater, organic compounds, anaerobic metabolism

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