基于磷脂脂肪酸的热带西太平洋沉积物生源要素矿化过程探析

doi:10.13745/j.esf.sf.2022.1.3

地学前缘 ›› 2022, Vol. 29 ›› Issue (4): 93-102.DOI: 10.13745/j.esf.sf.2022.1.3

• 深海生物地球化学过程 • 上一篇下一篇

基于磷脂脂肪酸的热带西太平洋沉积物生源要素矿化过程探析

杨梓阳¹^,²^,³^,⁴(), 任登龙⁵, 贺志鹏⁶^,^*(), 李学刚¹^,²^,³^,⁴^,^*(), 宋金明¹^,²^,³^,⁴, 袁华茂¹^,²^,³^,⁴, 段丽琴¹^,²^,³^,⁴, 李宁¹^,²^,³^,⁴, 张倩¹^,⁴

1.中国科学院海洋研究所中国科学院海洋生态与环境科学重点实验室, 山东青岛 266071
2.中国科学院大学, 北京 100049
3.青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室, 山东青岛 266237
4.中国科学院海洋大科学研究中心, 山东青岛 266071
5.中国石油化工股份有限公司胜利油田分公司海洋采油厂, 山东东营 257237
6.山东省淡水渔业研究院, 山东济南 250013

收稿日期:2021-09-10 修回日期:2021-11-15 出版日期:2022-07-25 发布日期:2022-07-28
通信作者: 贺志鹏,李学刚
作者简介:杨梓阳(1996—),男,硕士研究生,主要从事海洋生物地球化学研究。E-mail: yangziyang19@mails.ucas.ac.cn
基金资助:
国家自然科学基金项目(91958103);国家自然科学基金项目(42176200);中国科学院战略性先导科技专项(XDA23050501);山东省自然科学基金资助项目(ZR2020YQ28)

Exploring biomineralization in the tropical western Pacific sediments based on phospholipid fatty acid analysis

YANG Ziyang¹^,²^,³^,⁴(), REN Denglong⁵, HE Zhipeng⁶^,^*(), LI Xuegang¹^,²^,³^,⁴^,^*(), SONG Jinming¹^,²^,³^,⁴, YUAN Huamao¹^,²^,³^,⁴, DUAN Liqin¹^,²^,³^,⁴, LI Ning¹^,²^,³^,⁴, ZHANG Qian¹^,⁴

1. CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Science, Qingdao 266071, China
2. University of Chinese Academy of Science, Beijing 100049, China
3. Marine Ecology and Environmental Science Laboratory, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
4. Center for Ocean Mega-Science, Chinese Academy of Science, Qingdao 266071, China
5. Offshore Oil Production Plant of Sinopec Shengli Oilfield Company, Dongying 257237, China
6. Shandong Freshwater Fisheries Research Institute, Jinan 250013, China

Received:2021-09-10 Revised:2021-11-15 Online:2022-07-25 Published:2022-07-28
Contact: HE Zhipeng,LI Xuegang

摘要/Abstract

摘要：

甄别生源要素参与的海洋沉积物矿化过程对探析生源要素的生物地球化学循环有重要的作用,矿化作用包括有氧呼吸、硝酸盐还原、铁锰异化还原及硫酸盐还原等多个过程,但如何区分这些过程一直是海洋沉积物矿化研究的难点。本研究采用气相色谱-质谱(GC-MS)联用对热带西太平洋沉积物中的磷脂脂肪酸(phospholipid fatty acid, PLFA)的组成进行了解析,并分析不同矿化过程中的主要PLFA种类及其影响因素,探究PLFA对沉积物矿化的指示作用。结果表明,PLFA总量在有氧呼吸过程中最高,而在硝酸盐还原过程中最低;且14:0、i14:0、i15:0和i19:0是有氧呼吸过程中微生物PLFA的主要组成,当其含量明显降低时可以指示沉积物矿化从有氧呼吸转变为硝酸盐还原;而10:0、17:0、20:0和22:0含量之和显著增加时则指示了硫酸盐还原过程的发生。在热带西太平洋沉积物中,总有机碳(TOC)和总有机氮(TON)含量以及间隙水NO₃-N含量是PLFA含量的重要影响因素,PLFA总量随着TOC和TON含量的减少而减少,并且TOC和TON的降解能够促进PLFA降解的发生,对PLFA组成有更直接的影响。

关键词: 磷脂脂肪酸, 矿化过程, 指示作用, 海洋沉积物, 热带西太平洋

Abstract:

Identification of marine sediment mineralization processes plays an important role in the study of biogeochemical cycling of biogenic elements, although such processes—including aerobic respiration, nitrate reduction, iron and manganese reduction, sulfate reduction, etc. —can be difficult to distinguish one from another. In this study, gas chromatography-mass spectrometry (GC-MS) was used to analyze the phospholipid fatty acid (PLFA) profiles and their influencing factors in different mineralization processes, and further explore the use of PLFAs as sediment mineralization indicator in the tropical western Pacific. The total PLFA content was found to be highest in aerobic respiration and lowest in nitrate reduction. The obvious decrease of the sum of 14:0, i14:0, i15:0 and i19:0—the main components of microbial PLFA in aerobic respiration—could have certain indication for the occurrence of nitrate reduction; whilst the decrease of the sum of 10:0, 17:0, 20:0 and 22:0 could indicate the occurrence of sulfate reduction. In the sediments of the tropical western Pacific, the total organic carbon (TOC), total organic nitrogen (TON) and NO₃-N content in porewater were important factors influencing PLFA. as the total PLFA content decreased with decreasing TOC and TON, whilst the degradation of TOC and TON promoted the degradation of PLFA, which had a more direct impact on the PLFA composition.

Key words: phospholipid fatty acid, mineralization, indicative function, marine sediment, tropical western Pacific

中图分类号:

P736.2
P593

杨梓阳, 任登龙, 贺志鹏, 李学刚, 宋金明, 袁华茂, 段丽琴, 李宁, 张倩. 基于磷脂脂肪酸的热带西太平洋沉积物生源要素矿化过程探析[J]. 地学前缘, 2022, 29(4): 93-102.

YANG Ziyang, REN Denglong, HE Zhipeng, LI Xuegang, SONG Jinming, YUAN Huamao, DUAN Liqin, LI Ning, ZHANG Qian. Exploring biomineralization in the tropical western Pacific sediments based on phospholipid fatty acid analysis[J]. Earth Science Frontiers, 2022, 29(4): 93-102.

图/表 12

图1 热带西太平洋采样站位图

Fig.1 Location of the sampling station in the tropical western Pacific

表1 脂肪酸甲酯检出限、RSD和特征离子

Table 1 Detection limits, RSD and characteristic ions of fatty acid methyl esters

脂肪酸种类	检出限/(μg·mL^-1)	RSD/%	特征离子
8:0	0.032	2%	74 115 158
10:0	0.027	2%	74 143 186
12:0	0.023	2%	74 171 214
14:0	0.034	2%	74 199 242
i14:0	0.030	4%	74 211 242
15:0	0.044	3%	74 213 256
i15:0	0.033	4%	74 213 256
a15:0	0.032	4%	74 199 256
16:0	0.022	1%	74 227 270
i16:0	0.028	4%	74 227 270
17:0	0.031	2%	74 284 241
i17:0	0.033	4%	74 241 284
17:1ω7	0.039	3%	55 250 282
18:0	0.068	5%	74 298 255
i19:0	0.035	4%	143 269 312
20:0	0.053	4%	74 326 283
i20:1	0.035	4%	55 292 324
24:0	0.110	7%	74 382 339
13:0	0.022	1%	74 185 228
14:1	0.028	2%	55 208 240
16:1ω7	0.033	2%	55 236 268
i16:1	0.034	4%	55 194 236
18:1	0.071	5%	55 264 296
18:2	0.087	6%	67 294 263
20:3	0.077	5%	79 320 289
20:5	0.250	17%	79 287 316
22:1	0.080	5%	55 320 352
11:0	0.024	2%	74 157 200
20:4	0.093	6%	79 287 318

图2 脂肪酸甲酯色谱图

Fig.2 Chromatogram of fatty acid methyl esters

表2 微生物群落和PLFA生物标志物之间的对应关系[23⇓-25]

Table 2 Corresponding relationships between microbial groups and PLFA biomarkers [23⇓-25]

微生物	PLFA标志物
细菌	一些饱和或单不饱和脂肪酸:15:0,16:0,17:0,18:1ω5,18:1ω7,i19:0,a19:0
好氧细菌	16:1ω7,i14:0,a14:0,15:0 2OH,15:0 3OH,a15:0,i15:0
厌氧细菌	cy17:0,cy19:0
革兰氏阳性菌	a16:0,i16:0,a17:0,i17:0,i18:0
革兰氏阴性菌	i15:0 3OH,16:1ω9,i17:0 3OH
硫酸盐还原菌	17:1ω6,17:1ω7
放线菌	10Me16:0,10Me17:0,10Me18:0
假单胞菌	18:1ω7
真菌	18:3ω6 (6,9,12),18:1ω9

图3 沉积物中TOC、TON、TIN、TOC/TON、pH和δ13C的垂直变化

Fig.3 Vertical changes of sediment TOC, TON, TIN, TOC/TON, pH and δ13C

图4 沉积物间隙水中营养盐质量浓度的垂直变化

Fig.4 Vertical changes of nutrient concentration in sediment porewater

图5 沉积物中各种PLFA相对百分比

Fig.5 Relative percentages of various PLFAs in sediment

表3 沉积物中各种PLFA含量

Table 3 Content of various PLFAs in the sediment

深度/ cm	总 PLFA	各种PLFA含量/(μg·g^-1)
深度/ cm	总 PLFA	8:0	10:0	12:0	14:0	i14:0	i15:0	15:0	16:0	17:0	17:1ω7	18:0	i19:0	20:0	22:0	24:0	i16:1	i16:0	13:0	16:1ω7	i20:1	14:1	a15:0	i17:0	18:1	20:3	20:5	22:4	20:4	11:0	18:2	22:1
0~<1	0.27	0.002 7	0.001 2	0.001 5	0.011	0.024	0.007 1	0.004 4	0.046	0.005 0	0.013	0.012	0.012	0.003 7	0.005 6	0.007 2	-	0.039	0.001 3	0.038	0.005 0	0.001 4	0.001 7	0.004 0	0.008 0	0.002 3	0.002 7	0.003 2	-	0.000 64	0.002 0	0.004 0
1~<2	0.22	0.002 3	0.001 2	0.001 4	0.007 6	0.019	0.005 4	0.003 8	0.035	0.005 2	0.008 4	0.011	0.009 8	0.003 8	0.005 9	-	0.021	0.031	0.001 4	0.015	0.004 3	0.001 6	0.001 3	0.003 2	0.007 7	0.002 6	0.003 1	0.004 3	-	-	-	-
2~<3	0.20	0.002 3	0.001 1	0.001 2	0.007 1	0.017	0.004 9	0.003 4	0.032	0.004 6	0.007 0	0.009 1	0.003 6	0.003 4	0.005 4	0.007 0	0.018	0.027	0.001 2	0.025	-	0.001 4	0.001 2	0.002 8	0.006 7	0.002 3	0.002 8	0.003 9	0.003 2	-	-	-
3~<4	0.087	0.001 7	0.001 1	0.001 1	0.003 4	0.005 3	0.001 5	0.002 4	0.014	0.004 0	-	0.006 9	0.002 8	0.003 3	0.005 2	0.007 0	0.005 6	0.008 4	0.001 1	0.011	0.001 3	-	-	-	-	-	-	-	-	-	-	-
4~<5	0.028	0.001 4	0.000 89	0.000 89	0.001 6	0.000 54	0.000 17	0.001 7	0.004 8	0.003 3	-	0.004 7	0.000 29	0.002 7	0.004 5	-	-	0.000 59
5~<6	0.028	0.001 5	0.000 91	0.000 91	0.001 5	0.000 39	0.000 13	0.001 7	0.004 6	0.003 4	-	0.004 9	0.000 23	0.002 8	0.004 6	-	-	0.000 44
6~<7	0.032	0.001 4	0.000 86	0.000 86	0.001 5	0.000 38	0.000 11	0.001 6	0.004 5	0.003 1	-	0.004 5	0.000 13	0.002 6	0.004 3	0.005 9	-	0.000 32
7~<8	0.035	0.001 9	0.000 92	0.000 97	0.001 6	0.000 30	8.1E-05	0.001 8	0.004 5	0.003 5	-	0.005 0	0.000 11	0.002 9	0.004 8	0.006 6	-	-
8~<9	0.022	0.001 4	0.000 74	-	0.001 2	0.000 10	2.2E-05	0.001 4	0.003 4	0.002 9	-	0.004 0	2.2E-05	0.002 3	0.003 9	-	-	-
9~<10	0.021	0.001 2	0.000 68	-	0.001 2	0.000 13	3.3E-05	0.001 4	0.003 3	0.002 9	-	0.004 0	4.4E-05	0.002 3	0.003 8	-	-	-
10~<11	0.056	0.004 6	0.002 2	0.001 5	0.002 5	0.000 98	0.000 20	0.002 8	0.008 4	0.005 3	0.007 5	0.008 0	0.000 14	0.004 3	0.007 2	-	0.000 84	-
11~<12	0.039	0.003 0	0.001 3	0.001 4	0.002 1	0.000 27	5.7E-05	0.002 5	0.005 9	0.004 9	-	0.006 8	-	0.004 0	0.006 7	-	-	-
12~<13	0.043	0.002 3	0.001 5	-	0.001 9	0.000 21	5.2E-05	0.002 2	0.005 4	0.004 5	-	0.006 2	-	0.003 7	0.006 1	0.008 4	0.000 14	-
13~<14	0.044	0.002 2	0.001 2	0.001 1	0.001 7	0.000 27	6.0E-05	0.002 0	0.005 2	0.003 9	0.004 1	0.005 6	7.5E-05	0.003 3	0.005 4	0.007 4	-	-
14~<15	0.042	0.002 2	0.001 1	0.001 2	0.001 9	0.000 38	9.8E-05	0.002 2	0.005 5	0.004 2	-	0.006 1	9.8E-05	0.003 5	0.005 8	0.007 9	0.000 18	-
15~<16	0.043	0.002 0	0.001 1	0.001 1	0.001 8	0.000 22	6.2E-05	0.002 0	0.005 4	0.004 0	0.003 4	0.005 8	9.2E-05	0.003 4	0.005 6	0.007 6	-	-
16~<17	0.050	0.002 9	0.001 3	0.001 4	0.002 2	0.000 20	4.0E-05	0.002 6	0.006 3	0.005 1	-	0.007 2	3.9E-05	0.004 2	0.007 0	0.009 6	5.9E-05	-
17~<18	0.043	0.002 5	0.001 6	-	0.002 1	0.000 30	3.8E-05	0.002 5	0.005 8	0.004 9	0.005 7	0.006 8	3.8E-05	0.004 0	0.006 7	-	-	-
18~<19	0.046	0.002 8	0.001 4	0.001 3	0.002 0	0.000 27	5.4E-05	0.002 3	0.006 0	0.004 7	-	0.006 6	-	0.003 8	0.006 4	0.008 7	0.000 13	-
19~≤20	0.028	0.001 7	0.000 80	0.000 78	0.001 2	0.000 14	3.2E-05	0.001 4	0.003 5	0.002 8	-	0.004 1	-	0.002 3	0.003 8	0.005 3	-	-

表3 沉积物中各种PLFA含量

Table 3 Content of various PLFAs in the sediment

深度/ cm	总 PLFA	各种PLFA含量/(μg·g^-1)
深度/ cm	总 PLFA	8:0	10:0	12:0	14:0	i14:0	i15:0	15:0	16:0	17:0	17:1ω7	18:0	i19:0	20:0	22:0	24:0	i16:1	i16:0	13:0	16:1ω7	i20:1	14:1	a15:0	i17:0	18:1	20:3	20:5	22:4	20:4	11:0	18:2	22:1
0~<1	0.27	0.002 7	0.001 2	0.001 5	0.011	0.024	0.007 1	0.004 4	0.046	0.005 0	0.013	0.012	0.012	0.003 7	0.005 6	0.007 2	-	0.039	0.001 3	0.038	0.005 0	0.001 4	0.001 7	0.004 0	0.008 0	0.002 3	0.002 7	0.003 2	-	0.000 64	0.002 0	0.004 0
1~<2	0.22	0.002 3	0.001 2	0.001 4	0.007 6	0.019	0.005 4	0.003 8	0.035	0.005 2	0.008 4	0.011	0.009 8	0.003 8	0.005 9	-	0.021	0.031	0.001 4	0.015	0.004 3	0.001 6	0.001 3	0.003 2	0.007 7	0.002 6	0.003 1	0.004 3	-	-	-	-
2~<3	0.20	0.002 3	0.001 1	0.001 2	0.007 1	0.017	0.004 9	0.003 4	0.032	0.004 6	0.007 0	0.009 1	0.003 6	0.003 4	0.005 4	0.007 0	0.018	0.027	0.001 2	0.025	-	0.001 4	0.001 2	0.002 8	0.006 7	0.002 3	0.002 8	0.003 9	0.003 2	-	-	-
3~<4	0.087	0.001 7	0.001 1	0.001 1	0.003 4	0.005 3	0.001 5	0.002 4	0.014	0.004 0	-	0.006 9	0.002 8	0.003 3	0.005 2	0.007 0	0.005 6	0.008 4	0.001 1	0.011	0.001 3	-	-	-	-	-	-	-	-	-	-	-
4~<5	0.028	0.001 4	0.000 89	0.000 89	0.001 6	0.000 54	0.000 17	0.001 7	0.004 8	0.003 3	-	0.004 7	0.000 29	0.002 7	0.004 5	-	-	0.000 59
5~<6	0.028	0.001 5	0.000 91	0.000 91	0.001 5	0.000 39	0.000 13	0.001 7	0.004 6	0.003 4	-	0.004 9	0.000 23	0.002 8	0.004 6	-	-	0.000 44
6~<7	0.032	0.001 4	0.000 86	0.000 86	0.001 5	0.000 38	0.000 11	0.001 6	0.004 5	0.003 1	-	0.004 5	0.000 13	0.002 6	0.004 3	0.005 9	-	0.000 32
7~<8	0.035	0.001 9	0.000 92	0.000 97	0.001 6	0.000 30	8.1E-05	0.001 8	0.004 5	0.003 5	-	0.005 0	0.000 11	0.002 9	0.004 8	0.006 6	-	-
8~<9	0.022	0.001 4	0.000 74	-	0.001 2	0.000 10	2.2E-05	0.001 4	0.003 4	0.002 9	-	0.004 0	2.2E-05	0.002 3	0.003 9	-	-	-
9~<10	0.021	0.001 2	0.000 68	-	0.001 2	0.000 13	3.3E-05	0.001 4	0.003 3	0.002 9	-	0.004 0	4.4E-05	0.002 3	0.003 8	-	-	-
10~<11	0.056	0.004 6	0.002 2	0.001 5	0.002 5	0.000 98	0.000 20	0.002 8	0.008 4	0.005 3	0.007 5	0.008 0	0.000 14	0.004 3	0.007 2	-	0.000 84	-
11~<12	0.039	0.003 0	0.001 3	0.001 4	0.002 1	0.000 27	5.7E-05	0.002 5	0.005 9	0.004 9	-	0.006 8	-	0.004 0	0.006 7	-	-	-
12~<13	0.043	0.002 3	0.001 5	-	0.001 9	0.000 21	5.2E-05	0.002 2	0.005 4	0.004 5	-	0.006 2	-	0.003 7	0.006 1	0.008 4	0.000 14	-
13~<14	0.044	0.002 2	0.001 2	0.001 1	0.001 7	0.000 27	6.0E-05	0.002 0	0.005 2	0.003 9	0.004 1	0.005 6	7.5E-05	0.003 3	0.005 4	0.007 4	-	-
14~<15	0.042	0.002 2	0.001 1	0.001 2	0.001 9	0.000 38	9.8E-05	0.002 2	0.005 5	0.004 2	-	0.006 1	9.8E-05	0.003 5	0.005 8	0.007 9	0.000 18	-
15~<16	0.043	0.002 0	0.001 1	0.001 1	0.001 8	0.000 22	6.2E-05	0.002 0	0.005 4	0.004 0	0.003 4	0.005 8	9.2E-05	0.003 4	0.005 6	0.007 6	-	-
16~<17	0.050	0.002 9	0.001 3	0.001 4	0.002 2	0.000 20	4.0E-05	0.002 6	0.006 3	0.005 1	-	0.007 2	3.9E-05	0.004 2	0.007 0	0.009 6	5.9E-05	-
17~<18	0.043	0.002 5	0.001 6	-	0.002 1	0.000 30	3.8E-05	0.002 5	0.005 8	0.004 9	0.005 7	0.006 8	3.8E-05	0.004 0	0.006 7	-	-	-
18~<19	0.046	0.002 8	0.001 4	0.001 3	0.002 0	0.000 27	5.4E-05	0.002 3	0.006 0	0.004 7	-	0.006 6	-	0.003 8	0.006 4	0.008 7	0.000 13	-
19~≤20	0.028	0.001 7	0.000 80	0.000 78	0.001 2	0.000 14	3.2E-05	0.001 4	0.003 5	0.002 8	-	0.004 1	-	0.002 3	0.003 8	0.005 3	-	-

图6 各种PLFA与矿化过程的对照

Fig.6 Vertical variations of total PLFA and microbial PLFAs abundances in three types of sediment mineralization processes

表4 沉积物矿化过程及其化学反应[37]

Table 4 Sediment mineralization and related chemical reactions. Adapted from [37].

矿化过程	化学反应
有氧呼吸	(CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+138O₂→106CO₂+16HNO₃+H₃PO₄+122H₂O
硝酸盐还原	① (CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+94.4HNO₃→H₃PO₄+177.2H₂O+106CO₂+55.2N₂ ② (CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+84.8HNO₃→H₃PO₄+148.4H₂O+106CO₂+42.4N₂+16NH₃
铁还原	① (CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+212Fe₂O₃+848H⁺→H₃PO₄+530H₂O+106CO₂+16NH₃+424Fe²⁺ ② (CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+424FeOOH+848H⁺→424Fe²⁺+106CO₂+16NH₃+H₃PO₄+742H₂O
锰还原	(CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+472H⁺+236MnO₂→H₃PO₄+106CO₂+236Mn²⁺+8N₂+366H₂O
硫酸盐还原	(CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄) +53S $O 4 2 -$ →H₃PO₄+106H₂O+106CO₂+16NH₃+53S^2-

表4 沉积物矿化过程及其化学反应[37]

Table 4 Sediment mineralization and related chemical reactions. Adapted from [37].

矿化过程	化学反应
有氧呼吸	(CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+138O₂→106CO₂+16HNO₃+H₃PO₄+122H₂O
硝酸盐还原	① (CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+94.4HNO₃→H₃PO₄+177.2H₂O+106CO₂+55.2N₂ ② (CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+84.8HNO₃→H₃PO₄+148.4H₂O+106CO₂+42.4N₂+16NH₃
铁还原	① (CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+212Fe₂O₃+848H⁺→H₃PO₄+530H₂O+106CO₂+16NH₃+424Fe²⁺ ② (CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+424FeOOH+848H⁺→424Fe²⁺+106CO₂+16NH₃+H₃PO₄+742H₂O
锰还原	(CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄)+472H⁺+236MnO₂→H₃PO₄+106CO₂+236Mn²⁺+8N₂+366H₂O
硫酸盐还原	(CH₂O)₁₀₆(NH₃)₁₆(H₃PO₄) +53S $O 4 2 -$ →H₃PO₄+106H₂O+106CO₂+16NH₃+53S^2-

表5 PLFA与沉积物理化性质之间的相关性

Table 5 Correlation coefficients between sediment physio-chemical properties and PLFAs

PLFA种类	TON	TOC	TOC/TON	NO₃-N	NO₂-N	NH₄-N	pH值
总PLFA	0.933^**	0.942^**	0.769^**	0.698^**	0.464	0.198	-0.334
SSFA	0.822^**	0.840^**	0.692^**	0.622^**	0.346	0.162	-0.444
BSFA	0.955^**	0.956^**	0.774^**	0.674^**	0.515	0.179	-0.272
MUFA	0.951^**	0.958^**	0.779^**	0.740^**	0.485	0.228	-0.298
PUFA	0.910^**	0.945^**	0.796^**	0.747^**	0.433	0.257	-0.247

图7 沉积物中PLFA的PCA分析

Fig.7 PCA analysis of sediment PLFA

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基于磷脂脂肪酸的热带西太平洋沉积物生源要素矿化过程探析

Exploring biomineralization in the tropical western Pacific sediments based on phospholipid fatty acid analysis

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