地学前缘 ›› 2021, Vol. 28 ›› Issue (5): 159-166.DOI: 10.13745/j.esf.sf.2021.2.16

• 地下水污染修复 • 上一篇    下一篇

邻硝基对甲基苯酚和邻氨基对甲基苯酚对1,2,4-TCB厌氧生物降解影响研究

刘芷彤1(), 周妮2, 乔文静3, 叶淑君1,*()   

  1. 1.南京大学 地球科学与工程学院 表生地球化学教育部重点实验室, 江苏 南京 210023
    2.江苏环保产业技术研究院股份公司, 江苏 南京 210000
    3.南京农业大学 生命科学学院, 江苏 南京 210032
  • 收稿日期:2020-04-20 修回日期:2021-09-15 出版日期:2021-09-25 发布日期:2021-10-29
  • 通信作者: 叶淑君
  • 作者简介:刘芷彤(1995—),女,硕士研究生,主要从事地下水污染修复研究。E-mail: liuzhitong901@163.com
  • 基金资助:
    国家自然科学基金项目“地下水中氯代有机溶剂的厌氧生物研究”(42077174)

Effects of o-nitro-p-methylphenol and o-amino-p-methylphenol on the anaerobic biodegradation of 1,2,4-TCB

LIU Zhitong1(), ZHOU Ni2, QIAO Wenjing3, YE Shujun1,*()   

  1. 1. Ministry of Education Key Laboratory of Surficial Geochemistry, School of Earth Sciences and Engineering, Nanjing University, Nanjing 210023, China
    2. Jiangsu Academy of Environmental Industry and Technology Corp., Nanjing 210000, China 3. College of Life Sciences, Nanjing Agricultural University, Nanjing 210032, China
    3. College of Life Sciences, Nanjing Agricultural University, Nanjing 210032, China
  • Received:2020-04-20 Revised:2021-09-15 Online:2021-09-25 Published:2021-10-29
  • Contact: YE Shujun

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摘要：

南京某化工厂生产邻硝基对甲基苯酚和邻氨基对甲基苯酚,场地调查和室内微宇宙实验研究发现该场地发生了1,2, 4-三氯苯(1,2,4-TCB)污染,且场地内天然发生着1,2,4-TCB的厌氧还原脱氯反应。为探究该场地地下水中邻硝基对甲基苯酚和邻氨基对甲基苯酚对1,2,4-TCB厌氧脱氯过程的影响,开展了3组微宇宙实验:(1)底物仅为1,2,4-TCB, (2)底物为1,2,4-TCB和邻氨基对甲基苯酚,(3)底物为1,2,4-TCB和邻硝基对甲基苯酚。对比了3种不同底物条件下1,2,4-TCB的降解过程、降解速率及微生物群落结构。实验结果表明:(1) 邻硝基对甲基苯酚在厌氧条件下还原生成邻氨基对甲基苯酚, 同时抑制1,2,4-三氯苯厌氧生物降解;最终1,2,4-TCB降解产物只有1,4-DCB。(2)邻氨基对甲基苯酚不会阻碍1,2,4-TCB的降解,但会降低降解速率。(3) Azospira和Pseudomonas与邻硝基对甲基苯酚还原反应有关,尤其是Pseudomonas极有可能是场地邻硝基对甲基苯酚还原的功能菌。

关键词: 邻硝基对甲基苯酚, 邻氨基对甲基苯酚, 1,2,4-TCB, 厌氧生物降解, 微生物群落

Abstract:

In a Nanjing chemical plant manufacturing o-nitro-p-methylphenol and o-amino-p-methylphenol, field investigation and microcosm study found contaminated groundwater where the contaminant, 1,2,4-trichlorobenzene (1,2,4-TCB), was biodegraded by indigenous anaerobic microbes. In this study, we conducted three groups of microcosm experiments to explore the effects of o-nitro-p-methylphenol and o-amino-p-methylphenol on the 1,2,4-TCB dechlorination process, using (1) 1,2,4-TCB alone, (2) 1,2,4-TCB and o-amino-p-methylphenol, or (3) 1,2,4-TCB and o-nitro-p-methylphenol as the substrate(s), and compared the degradation processes, degradation rates, and microbial communities for the three groups of experiments. The results show: (1) O-nitro-p-methylphenol was reduced to o-amino-p-methylphenol while 1,2,4-TCB dechlorination was inhibited, with 1,4-DCB as the only dechlorination product. (2) O-amino-p-methylphenol did not prohibit 1,2,4-TCB degradation but decreased degradation rate. (3) Azospira and Pseudomonas were involved in o-nitro-p-methylphenol reduction, especially Pseudomonas was probably the functional microorganism under the anaerobic condition at the site.

Key words: o-nitro-p-methylphenol, o-amino-p-methylphenol, 1,2,4-TCB, anaerobic biodegradation, microbial community

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