Pollutant distribution and microbial characteristics at a petrochemical site in the Yangtze River Economic Belt

doi:10.13745/j.esf.sf.2022.1.46

Abstract

Abstract:

Soils at different depths were collected from three sampling points in an in-service petrochemical site in the Yangtze River Economic Belt. Thereafter, the concentration of petroleum hydrocarbons and the microbial community structure in the samples were measured to reveal the pollutant distribution and microbial characteristics. The overall pollution degree of the three points was found to differ; however, the pollutant concentration first increased and then decreased with depth in all cases, with the highest concentration found at the depth of approximately 4.0 m (i.e., near the groundwater level). C6-C9 up to 5302 mg/kg, C10-C40 up to 625.6 mg/kg, aromatic hydrocarbon up to 455.2 mg/kg, and halogenated hydrocarbons up to 41.54 mg/kg were detected in the soils. The richness and diversity of soil microorganisms were found to decrease with an increase in soil depth, and at the same depth, the richness and diversity of the soil microorganisms decreased with an increase in soil pollutant concentration. At the phylum level, the relative abundances of Actinobacteria, Firmicutes, and Spirochaetes were significantly positively correlated with the concentration of petroleum hydrocarbon (p<0.05). Further, at the genus level, the relative abundances of Streptomyces, SCADC1-2-3, Desulfitobacterium, and Cryptanaerobacter were significantly positively correlated with the concentration of petroleum hydrocarbon (p<0.05).

Key words: petrochemical site, soil, petroleum hydrocarbon, microorganisms, community structure

CLC Number:

S154
X53

YE Xiangyu, CHEN Xuexia, YU Bo, ZHANG Min, GUO Caijuan, LU Xiaoxia. Pollutant distribution and microbial characteristics at a petrochemical site in the Yangtze River Economic Belt[J]. Earth Science Frontiers, 2022, 29(3): 239-247.

Figures/Tables 7

References 27

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点位	样品编号	深度/m	有效序列数	OTU数/Richness	Chao1	Shannon_e	Simpson
A	A-2.4	2.4	38 846	2 017	2 019.9	5.75	0.010
A	A-4.0	4.0	33 155	1 387	1 390.3	4.41	0.051
A	A-4.5	4.5	44 892	1 467	1 470.5	4.43	0.033
A	A-5.5	5.5	49 012	1 122	1 125.2	2.91	0.171
A	A-7.5	7.5	31 459	596	605.6	2.64	0.314
B	B-2.4	2.4	36 422	2 145	2 147.3	5.89	0.009
B	B-4.0	4.0	29 742	1 117	1 121.4	3.53	0.142
B	B-4.5	4.5	41 625	970	974.2	3.02	0.133
B	B-5.0	5.0	28 294	1 095	1 099.7	3.38	0.134
B	B-7.5	7.5	46 683	795	801.0	2.29	0.273
C	C-3.2	3.2	48 404	1 290	1 294.2	3.70	0.105
C	C-4.0	4.0	49 236	843	847.5	2.23	0.319
C	C-5.0	5.0	52 117	1 341	1 344.1	2.95	0.161
C	C-7.5	7.5	49 358	765	770.4	3.95	0.054

点位	样品编号	深度/m	有效序列数	OTU数/Richness	Chao1	Shannon_e	Simpson
A	A-2.4	2.4	38 846	2 017	2 019.9	5.75	0.010
A	A-4.0	4.0	33 155	1 387	1 390.3	4.41	0.051
A	A-4.5	4.5	44 892	1 467	1 470.5	4.43	0.033
A	A-5.5	5.5	49 012	1 122	1 125.2	2.91	0.171
A	A-7.5	7.5	31 459	596	605.6	2.64	0.314
B	B-2.4	2.4	36 422	2 145	2 147.3	5.89	0.009
B	B-4.0	4.0	29 742	1 117	1 121.4	3.53	0.142
B	B-4.5	4.5	41 625	970	974.2	3.02	0.133
B	B-5.0	5.0	28 294	1 095	1 099.7	3.38	0.134
B	B-7.5	7.5	46 683	795	801.0	2.29	0.273
C	C-3.2	3.2	48 404	1 290	1 294.2	3.70	0.105
C	C-4.0	4.0	49 236	843	847.5	2.23	0.319
C	C-5.0	5.0	52 117	1 341	1 344.1	2.95	0.161
C	C-7.5	7.5	49 358	765	770.4	3.95	0.054

组分	各组分间的相关系数
组分	P1	P2	P3	P4		P5		P6		P7	P8		P9	P10	P11		C6-C9	C10-C40
P1	1.000
P2	-0.916^**	1.000
P3	-0.776^**	0.692^**	1.000
P4	-0.349	0.284	-0.108	1.000
P5	0.037	-0.090	-0.147	0.174	1.000
P6	-0.332	0.231	0.055	0.754^**	0.015		1.000
P7	0.279	-0.174	-0.125	-0.020	0.490		0.059		1.000
P8	-0.604^*	0.442	0.560^*	0.121	0.099		0.165		-0.218		1.000
P9	-0.029	0.059	-0.015	-0.015	0.499		0.020		0.424		-0.103	1.000
P10	-0.055	-0.112	0.110	0.416	-0.242		0.279		-0.268		0.320	-0.643^*		1.000
P11	-0.099	0.196	-0.103	0.569^*	0.486		0.363		0.310		-0.024	0.059		0.220		1.000
C6-C9	-0.422	0.298	0.678^**	-0.280	-0.136		-0.060		-0.167		0.642^*	-0.073		0.157		-0.444	1.000
C10-C40	-0.726^**	0.726^**	0.757^**	-0.141	0.031		-0.185		-0.130		0.581^*	0.009		-0.037		-0.145	0.724^**	1.000

组分	各组分间的相关系数
组分	P1	P2	P3	P4		P5		P6		P7	P8		P9	P10	P11		C6-C9	C10-C40
P1	1.000
P2	-0.916^**	1.000
P3	-0.776^**	0.692^**	1.000
P4	-0.349	0.284	-0.108	1.000
P5	0.037	-0.090	-0.147	0.174	1.000
P6	-0.332	0.231	0.055	0.754^**	0.015		1.000
P7	0.279	-0.174	-0.125	-0.020	0.490		0.059		1.000
P8	-0.604^*	0.442	0.560^*	0.121	0.099		0.165		-0.218		1.000
P9	-0.029	0.059	-0.015	-0.015	0.499		0.020		0.424		-0.103	1.000
P10	-0.055	-0.112	0.110	0.416	-0.242		0.279		-0.268		0.320	-0.643^*		1.000
P11	-0.099	0.196	-0.103	0.569^*	0.486		0.363		0.310		-0.024	0.059		0.220		1.000
C6-C9	-0.422	0.298	0.678^**	-0.280	-0.136		-0.060		-0.167		0.642^*	-0.073		0.157		-0.444	1.000
C10-C40	-0.726^**	0.726^**	0.757^**	-0.141	0.031		-0.185		-0.130		0.581^*	0.009		-0.037		-0.145	0.724^**	1.000